Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.

建立简有的木瓜凝乳蛋白酶分离纯化方法,究其体外溶核用。

Objective To develop a simple and effective m etho d for the separation and purification of chymopapain and study the zymological p roperty of the enzyme.

目的建立简有的木瓜凝乳蛋白酶分离纯化方法,究其酶学性质。方法采用离子交换层析直接将木瓜凝乳蛋白酶分离纯化,通过温度、葡萄糖、盐酸胍及其他因素究酶学性质。