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Abstract: Aim:To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis.

摘 要: 目的:建立显微受精的方法,并探讨睾精子在显微注射进入卵母后的受精能力。

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Conclusion The electrofusion efficiency may he improved by inserting more somatic cells into PVS when the somatic cells have a low electrofusion efficiency with oocytes.

结论低融合效率的体胞与卵母电融合时可通过增加PVS中体胞的数目来提高融合效率。

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Then their synthese speed slows down.But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.

上皮胞在卵母卵黄形成期,它的RNA和DNA的合成均较快,因此认为上皮胞合成分泌了一些物质参与卵黄蛋白的形成。

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The secondary oocyte now divides again into two new cells of unequal size: a relatively large ootid (mature egg cell) and a minute second polar body.

此时,次级卵母又分裂成两个大不对等的新胞:一个相对很大的卵子(成熟卵胞)和一个微的次级极体。

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双语版 TED-Ed 演讲精选

The tiny oocyte moves along the fallopian tube.

微小的卵母细胞沿输卵管运动。

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Osmosis-解剖与生理

The primary oocyte has 46 chromosomes, but eventually it has to turn into a gamete with only 23 chromosomes.

初级卵母细胞有46条染色体,但是最终会变成只有23条染色体的配子。

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Osmosis-生殖

So, during ovulation, the follicle ruptures and releases the egg, which inadvertently leads to epithelial cell damage.

在排卵过程中,卵泡破裂,卵母细胞释出,也无意中导致了上皮细胞的损伤。

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Osmosis-生殖

And, each follicle is made up of a germ cell, also known as an oocyte, which is the immature egg.

卵泡都生殖细胞组成,也就是卵母细胞,就是未成熟的卵子。

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Osmosis-解剖与生理

After ovulation, the secondary oocyte makes a very quick journey through the peritoneal space and lands in the fallopian tube.

排卵后,次级卵母细胞会快速地通过腹膜,最后落入输卵管。

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Osmosis-解剖与生理

Meanwhile, the primordial follicle turns into a primary follicle, meaning that the follicular cells that surrounding the primary oocyte develop into granulosa cells.

,包围初级卵母细胞的滤泡细胞分化为颗粒细胞,使始基卵泡变为初级卵泡。

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TED-Ed(视频版)

This inner machinery includes two ovaries stocked with thousands of tiny sacks called follicles that each contain one oocyte, an unfertilized egg cell.

这套内部机制包含两卵巢,卵巢内存有成千上万的微小袋子,叫做卵泡,每卵泡内都含有卵母细胞,即未受精卵细胞。

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Osmosis-解剖与生理

Each primordial follicle has a single immature sex cell called the primary oocyte at the core, and a layer of follicular cells surrounds this.

始基卵泡中心都有未成熟的生殖细胞,叫做初级卵母细胞,被卵泡细胞包裹。

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Osmosis-解剖与生理

The first stage lasts from infancy to puberty, and during this stage the primary oocyte remains stuck in the prophase step of meiosis 1.

阶段从婴儿期到青春期,初级卵母细胞还停留在第次减数分裂的前期。

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Osmosis-解剖与生理

These two phases are separated by ovulation, which is when the follicle ruptures and releases an oocyte that is ready to be fertilized.

这两阶段被排卵分开,排卵即:卵母细胞被卵泡排出,准备好受精。

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Osmosis-解剖与生理

This triggers the primary oocyte within the dominant follicle to finally complete meiosis 1, and turn into a secondary oocyte, which has 23 chromosomes.

这使得优势卵泡里的初级卵母细胞最终完成第次减数分裂,变成了有23条染色体的次级卵母细胞

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Osmosis-解剖与生理

If fertilization occurs - meaning if a sperm meets the secondary oocyte - then the corpus luteum continues making progesterone until the placenta forms.

如果这候受精,精子遇到次级卵母细胞,黄体会持续地分泌黄体酮,直到胎盘形成。

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Osmosis-解剖与生理

To do this, the primary oocytes have to complete meiosis 1, and in a person's lifetime only about 400 successfully do that.

要变成配子就必须要经历第次减数分裂,而人的生中大概只有400初级卵母细胞可以做到。

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Osmosis-生殖

At ovulation, the oocyte is released into the fallopian tube, and the luteal phase begins - which lasts for the last 2 weeks of an average 28-day cycle.

排卵卵母细胞被释放入输卵管,然后黄体期就开始了,它会占据月经周期的后两周。

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Osmosis-生殖

Each follicle is initially made up of an immature sex cell, or primary oocyte, which is the female sex cell, and layers of theca and granulosa cells surrounding the oocyte.

卵泡最初是未成熟的生殖细胞:初级卵母细胞(即女性生殖细胞)和卵母细胞外层的鞘细胞、颗粒细胞组成。

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Osmosis-解剖与生理

The first part is the fimbriae which are the finger-like projections that surround the ovary and guide the secondary oocyte into the fallopian tube.

首先经过的是输卵管的伞部,呈指状突起,包围着卵巢,并且可以引导次级卵母细胞进入输卵管。

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Osmosis-解剖与生理

If fertilization doesn't happen, then the corpus luteum stops making hormones after around 10 days, becomes fibrotic, and is called the corpus albicans.

如果次级卵母细胞没有受精,大概10天后,黄体就会停止释放激素而纤维化,形成白体。

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Osmosis-生殖

The fourth subtype is a dysgerminoma and it's made of germ cells that turn into oocytes, which is the normal pattern, but then they start to grow uncontrollably.

第四种亚型是无性细胞瘤,它是转化为卵母细胞的生殖细胞组成,虽然这是正常发育模式,但后它们开始增长失控。

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Osmosis-解剖与生理

In a graafian follicle, a central cavity called the antrum forms within the follicle, and the granulosa cells secrete a nourishing fluid for the primary oocyte directly into that antrum.

格拉夫卵泡的中间会形成空腔叫做卵泡腔,而颗粒细胞会为初级卵母细胞分泌营养液,直接排入卵泡腔。

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Osmosis-解剖与生理

On the inside, the fallopian tubes have smooth muscle with an inner lining that has ciliated cells that slowly sweep the secondary oocyte or zygote towards the uterus.

输卵管内是有纤毛细胞覆盖的平滑肌,这些纤毛可以缓慢地将次级卵母细胞或者受精卵向子宫运输。

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