A method for simultaneous separation and determination of two coumarins,dicumarol and cyclocoumarol,in cosmetics by HPLC-diode-array detector(DAD) was developed.
Tetrachloroethane in air was collected by activated carbon,desorped with carbon disulfide,separated by GDX-103 chromatography column,and detected by flame ionization detector.
So the absorbing wavelength is selected,the operating condition optimized,and the quantitative and qualitative determination method on Polyoxin B by HPLC-DAD established.
A method based on the resistance-displacement relationship of shape memory alloy(SMA) spring that can adjust the nonaxiality of small-diameter pipe inner surface inspection sensor is proposed.
The coloring matter are separated by YWG-C18,the best wave is found by DAD detecter, the quantitative method is standard curve , the result is accurately ,the method are used simply.