Tigogenin was separated completely from the crude sapogenins by macroporous resin column chromatography,only simple solvent Ilelute was used in the column of the separate process.

用大孔树脂剂的色谱方法在离的过程中仅需要简单的溶剂-Ⅱ,离出的单一组剑麻皂甙元占粗皂甙元的比率高达70%以上。

The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured.

经金属螯合亲层析柱(Ni-NTA agarose)纯胶柱上在位复性后，ELISA结果证实其具有良好的结合β3抗原活性。