1.Age-related alteration in hepatic acyl-CoA: cholesterol acyltransferase and its relation to LDL receptor and MAPK.

1.（与年龄相关辅酶A改变：胆固醇转移酶及其与LDL受体和MAPK关系）。

2.To remove bound CoA from the protein, the protein solution was buffer exchanged into 10 mM citrate (pH 5.0)–1 mM TCEP by using an S1Y10 spiral cartridge (Millipore).

2.删除约束，农委会从蛋白质，蛋白质解决法缓冲交换到10毫米，柠檬酸（ pH值5.0 ） -1毫米tcep使用s1y10螺旋墨盒（ millipore ） 。