This is constantly replenished, with around 500 ml of new CSF produced everyday and the excess, or 500 minus 150 mL or 350 mL, is absorbed into the blood.
The CSF fills the space that surrounds the brain and wastes from inside their brain make their way out to the CSF which gets dumped along with the waste into the blood.
The diagnosis of west nile virus is made by detecting about four times the normal amount of IgM in the serum or CSF using an immunoassay test that's specific to the virus.