The main equipment here is a thermal cycler system for nucleic acid amplification (PCR, quantitative fluorescence detection or common).
主就是核酸扩增循环(PCR,实时普通的)。
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Throw everything in the thermal cycler, wave your magic wand, and the process begins.
把所有成分加入热循环仪,挥动" 魔杖" ,这个过程就开始了。
Take it from Jill Warren, she's a lifelong cycler and the CEO of the European Cyclists' Federation.
从吉尔·沃伦 (Jill Warren) 那里拿来吧,她一名终车手,也欧车联合会的首席执官。
So first of all, we're going to need a machine called a thermal cycler - that's where the PCR magic happens.
所以首先,我们需要一个叫做热循环仪的仪器,这PCR发生的地方。
It requires a thermal cycler, filled with the DNA sample to be multiplied, thermostable Taq polymerase, primers with which we select what gets multiplied, and free nucleotides of all 4 types - A, T, C and G.
它需要往热循环仪中加入用于扩增的DNA样本、耐热的Taq聚合酶、扩增时选用的引物,以及所有4种类型的游离核苷酸A、T、C和G。
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