1.Methods By means of bicolour fluorescence and flow cytometry (FCM) analysis, the results were compared during different lay-out time, hemolysis time and washing time.

1.方法用双抗双色免疫荧光标，细胞仪分析，对标本放置不同时间、溶血后洗涤后的结果进行比较。

2.In vitro antigen presentation assays, in vivo administration of the bromodeoxyuridine coupled to flow cytometry assays assessed intra-islet T cell activation in the absence or presence of B cells.

2.离实验测定抗原呈递活；动物实验，用溴脱氧尿苷处理后以细胞术检测B细胞（存或不存时）对胰岛内T细胞活的影响。