Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product.

释放酸脱氢酶存在于培养物上清中,可用酶联检法30分钟量。这种酶联分析导致四唑盐（INT）转化成红甲臜化合物。成量与裂解细胞数目成正比。