Any blood vessels encountered along the way are ligated.

操作过程中遇到任何血管都要扎。

Transfer of the ligated plasmid into an E. coli strain (transformation).

7。转化成功细菌筛选。

They found that it wasn't necessary to ligate a dog's pancreatic ducts in order to collect insulin.

们发现没有必要扎管来收集岛素。

Open, identify, ligate, remove, irrigate, close.

打开，确认，扎，移出，冲洗，缝合。

It read, " Ligate pancreatic ducts of dogs." Banting's idea was to close off the small passages in a dog's pancreas that release its products into the rest of its body.

上面写着：“扎管。”班廷想法是关闭腺中小通道，这些通道将其产物释放到身体其部位。