2.A mutant, TS-N-121 was obtained by mutation and plate screening from a parent strain Mycobacterium fortuitum MF2 , which could transform sterol into androstanedione as the main product.
1.Most modern eukaryotes rely on fat-like compounds called sterols, such as cholesterol, to build cell membranes and carry out other cellular functions.
3.Because sterols are found throughout the eukaryotic family tree, they are thought to have been present in the last common ancestor of all modern eukaryotes.
5.One possibility is that eukaryotes that make more-modern sterols gained a selective advantage between one billion and 800 million years ago, eventually displacing their protosterol-making counterparts.
7.Benjamin Nettersheim, a geobiologist at the University of Bremen in Germany, Jochen Brocks, a palaeobiogeochemist at the Australian National University in Canberra, and their colleagues decided to focus on short-lived molecules that modern eukaryotes make while synthesizing sterols.