Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.

方法取胎分离成纤维细胞,分别采用细胞悬液法和酶消化植培养法对其进行培养。

Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.

方法 标本用冷消化法分离出尿路上皮细胞，培养于无血清生长培养基。