These molecules are smaller than the wavelength of light, which means that we can never see them directly, even with the best light microscopes.

这些分子比光的波长还小，也就是说，我们永远不能直接看它们，即使用最先进的光学微镜也做不到。

On light microscopy, there'll often be mesangial proliferation, or expansion of the mesangium, and upon getting a closer look with electron microscopy, there'll be immune complexes deposited within the mesangium.

光学微镜下，常常膜增生或膜扩张，进一步电镜下观察，免疫复合物沉积于膜上。

It's also too tiny to be seen with a light microscope, so to visualize it, you need a darkfield microscope with use of immunofluorescence is required.

这种细菌也因为体型太细而难以在光学微镜下像，所以为了能够看钩端螺旋体，人们需要使用暗视野微镜以及荧光染色剂。

We start our investigations at a very small magnification, which is equivalent to the highest magnification, which you can actually reach with a light microscope.

我们从一个很小的放大倍率开始我们的研究，这相当于你用光学微镜能达到的最高放大倍率。

The invention of the visible-light microscope late in the sixteenth century introduced a previously unknown realm of single-celled plants and animals.

16世纪，光学微镜成功，此项开了人们对未知动植物的了解。

He designed his own light microscope, which used multiple glass lenses to light and magnify specimens.

他设计了自己的光学微镜，使用多个玻璃透镜来照亮和放大标本。

This image was taken with a light microscope.

这张图片是用光学微镜拍摄的。

During the 19th century, huge medical progress was made possible by the light microscope.

在19世纪，光学微镜使得医学取得了巨大的进步。

They incorporate optical sectioning using high-resolution confocal microscopy.

他们采用高分辨率共聚焦微镜进行光学切片。

An optical microscope, with a typical resolution of about 250 nanometres, can then produce images of individual elements within cells that are, in reality, only 60 nanometres in width.

典型分辨率约为 250 纳米的光学微镜以生成细胞内单个元素的图像，而实际上这些元素的宽度仅为 60 纳米。

They monitored changes in these cracks under increasing pressure using optical and electron microscopes And found that the cracks barely grew until the force used exceed 500 Newtons.

他们使用光学微镜和电子微镜监测这些裂纹在不断增加的压力下的变化，并现直到施加的力量超过500牛顿时，裂纹才开始增长。

For centuries, scientists have been limited by the fact that optical microscopes couldn't achieve a resolution smaller than 0.2 micrometers, or half the wavelength of visible light.

几个世纪以来， 光学微镜无法达到小于 0.2 微米（ 或光波长的一半） 的分辨率这一事实限制了科学家们。

So maybe this sounds like a super stupid question, but why can't you just use a regular light  microscope to take a picture of a protein?

这或许听起来是个极其愚蠢的问题，但为何不能用普通光学微镜来拍摄蛋白质的图像呢？

These vital components of life are too small to be seen by the naked eye, or even by a light microscope.

生命中的这些重要组分小到肉眼甚至光学微镜都无法看。

Even the best light microscopes can't see anything much smaller than about 200 nm, because, well, light can't really interact with something smaller than its wavelength.

即使是最好的光学微镜也无法看小于 200 纳米的任何东西，因为光实际上无法与小于其波长的物体相互作用。

But they still have their limits—modern optical microscopes cannot image structures smaller in size than the wavelength of visible light, or a few hundred nanometres.

但它们仍有其局限性——现代光学微镜无法对尺寸小于光波长或几百纳米的结构进行成像。

When we zoom in on its wing using a light microscope, we see that it's composed of tiny overlapping scales.

当我们用光学微镜放大其翅膀时，以看到它是由许多微小的重叠鳞片组成的。

Well-differentiated tumors with adenocarcinoma or squamous cell carcinoma morphology can be diagnosed by routine light microscopy, without the need for IHC.

具有腺癌或鳞状细胞癌形态的分化良好的肿瘤以通过常规光学微镜进行诊断，而不需要 IHC。