Methods The sciatic and brachial plexus nerve of three-day-old SD mice was treated with collagenase type IV and trypsin to remove the desmocyte on the Schwann cell, then explanted in the culture disk.
先用IV型胶胰蛋白消化出生3天SD鼠臂层神坐骨神块后再将块贴壁培养。
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