Immunologic characteristics of LPP antigen in this patient were investigated by indirect immunofluorescence immunoblotting and compared with bullous pemphigoid (BP) antigen.
A molecularly imprinted solid phase extractant, particularly for the recognition of diphenylguanidine (DPG) was prepared using diphenylguanidine (DPG) as the template and bulk polymerization.
But, to be sure the immune-shielding proteins were still there, the team used two special techniques, immunofluorescence and western blotting, to check for their presence.