Protein response, 24-hour nitrogen balance, but that second meal would be just about getting your protein in.

蛋白质反应， 24小时氮平衡，但第二餐主了摄取蛋白质。

The current RDA which is a minimum to prevent a deficiency is based on nitrogen balance.

目前的RDA基氮平衡来防止缺乏症的最。

And one must understand that this came from nitrogen balance studies and typically those are young men, 18-year-old men.

必须明白这一点源自氮平衡研究， 而这些研究通常针对的年轻男性，即18岁的男性。