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Method Bifidobacterium plasmid was extracted by mutanolysin from Streptomyces globisporus which could lyse the wall of bifidobacterium.The result was compared with that by lysozyme.

方法利用球孢链霉菌发酵产生的变溶菌化双歧杆菌壁,提取质粒,并与商业化溶菌酶法比较。

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NPC cell line (CNE1) as target,Electroporation was used to transfect EBV-LMP gene and the vector into CNE1 cells.

以人高分化鼻咽癌(CNE1)为对象,采用电穿孔基因转染技术,将重组EBV-LMP表达质粒转染CNE1

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Result Both mutanolysin and lysozyme could successfully lyse the wall of bifidobacterium and extract the plasmid from Bifidobacterium.

结果球孢链霉菌发酵液与溶菌酶均成功提取到质粒

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经济学人-科技

Some are exchanged via small loops of DNA called plasmids.

基因会通过被称为质粒DNA小环交换。

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博兹曼科学——生物

They also have extra little tiny loops called plasmids.

它们还具有额外小环,称为质粒

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2022诺贝尔得主访谈录

I did all these experiments actually, with my own hands, I was 58 years old, I was still culturing plasmids and feeding cells, so it is very unlikely.

际上,所有验都是我亲手做,当时我 58 岁,我还在培养质粒和喂养细胞,所以是非常不可能

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Osmosis-微生物

However, plasmids often contain genes that confer a selective advantage to the bacterium such as resistance to antibiotics.

不过质粒通常包赋予细菌选择性优势基因,例如抗生素耐药性。

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Osmosis-微生物

The cytoplasm contains ribosomes which are the site of protein synthesis, the nucleoid which contains the genetic material, and plasmids which contain genes that confer a selective advantage to the bacterium.

细胞质中有核糖体,它们是蛋白质合成部位;拟核有遗传物质以及质粒质粒有赋予细菌选择性优势基因。

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Osmosis-微生物

Plasmids have relatively few genes and their genetic information is not essential to the bacterium and cells that lack them usually function normally.

质粒基因相对较少,并且它们基因对细菌来说并不是,缺少质粒细胞往往也可以正常生长。

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Osmosis-微生物

Additionally to the genetic material contained within the nucleoid, many bacteria contain extra chromosomal DNA molecules called plasmids.

除了包在拟核中遗传物质外,许多细菌有额外DNA分子,称为质粒

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Did You Know? – Verge Science

Then they dropped the plasmid into a living bacterium, or yeast.

然后他们将质粒放入活细菌或酵母中。

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Paper Talk

They loaded it with plasmid's DNA, carrying the CRR gene, and placed this gel right into the brain cavity after removing a glial blastoma tumor in mice.

他们将携带CRR基因质粒DNA装入其中,然后在小鼠切除胶质母细胞瘤后,将种凝胶直接植入脑腔。

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SciShow 合集

But this is the first time the plasmid has been found in the United States, and it could get passed around quickly.

还是第一次在美国发现质粒,它可能会迅速传播开来。

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2017级专外有声

Vectors also incorporate a selectable marker, a gene which allows host cells containing the vector to be selected from amongst those which do not, usually by conferring resistance to a toxin, or enabling their survival under certain growth conditions.

最初大肠杆菌载体是染色体外环形质粒,并且相当数量噬菌体(感染细菌病毒)也被用在大肠杆菌中。

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Life Noggin

So essentially, these plasmids are the vehicles that transfer genes for antibiotic resistance between different bacterial species.

因此本质上,质粒是在不同细菌物种之间转移抗生素抗性基因载体。

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语料库

Soon after, others began using plasmid vectors and adding genes for antibiotic resistance, greatly increasing the reach of the recombinant techniques. [81]

不久之后,其他人开始使用质粒载体并添加抗生素抗性基因,大大增加了重组技术应用范围[81]。

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Writing in the Sciences

This one had cell culture and transfections, antibodies, plasmids, recombinant virus production and infection, and so on and so forth.

个有细胞培养和转染、抗体、质粒、重组病毒生产和感染等等。

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SciShow 合集

And the thing that makes this mutation especially troubling is that it was found on a plasmid -- a little piece of DNA that can be easily shared between neighboring bacteria, meaning it can spread more easily.

种突变特别令人担忧是,它出现在一个质粒上——是一种可以轻易在邻近细菌之间共享小段DNA,意味着它可以更容易地传播。

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Life Noggin

Or, in plain english, plasmids are part of the reason that, one day, antibiotics may not be able to help your strep throat anymore.

或者,用简单英语来说,质粒是有一天抗生素可能无法再帮助您治疗链球菌性咽喉炎部分原因。

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博兹曼科学——生物

And so a plasmid, remember this would be the chromosomal DNA. This would be the chromosome of the bacteria.

因此,质粒,记住将是染色体DNA。将是细菌染色体。

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2017级专外有声

More specialized vectors have been engineered to use aspects of plasmids and bacteriophages, such as the plasmid-bacteriophage h hybrids known as cosmids

质粒和噬菌体已经在很多种类细菌中被使用来表达基因,并且一噬菌体也许已经被用来将DNA整合到宿主基因组上。

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博兹曼科学——生物

We're just transferring that DNA, that plasmid from one to another.

我们只是将DNA,即质粒,从一个转移到另一个。

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SciShow 合集

This plasmid mutation has been seen in other places before; in China, in animals and some human patients.

质粒突变之前在其他地方出现过;在中国,出现在一动物和人类患者身上。

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