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Method Bifidobacterium plasmid was extracted by mutanolysin from Streptomyces globisporus which could lyse the wall of bifidobacterium.The result was compared with that by lysozyme.

方法利用球孢链霉菌发酵产生的变菌素来消双歧杆菌细胞壁,提取质粒,并业菌酶法比较。

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NPC cell line (CNE1) as target,Electroporation was used to transfect EBV-LMP gene and the vector into CNE1 cells.

以人高分鼻咽癌细胞株(CNE1)为对象,采用电穿孔基因转染技术,将重组EBV-LMP表达质粒转染CNE1细胞。

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Result Both mutanolysin and lysozyme could successfully lyse the wall of bifidobacterium and extract the plasmid from Bifidobacterium.

结果球孢链霉菌发酵液菌酶均成功提取到质粒。

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经济学人-科技

Some are exchanged via small loops of DNA called plasmids.

一些基因会通被称为的DNA小环交换。

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博兹曼科学——生物

They also have extra little tiny loops called plasmids.

它们还具有额外的小环,称为。

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2022诺贝尔得主访谈录

I did all these experiments actually, with my own hands, I was 58 years old, I was still culturing plasmids and feeding cells, so it is very unlikely.

实际上,所有这些实验都是我亲手做的,当时我 58 岁,我还在培养和喂养胞,所以这是非常不可能的。

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Osmosis-微生物

However, plasmids often contain genes that confer a selective advantage to the bacterium such as resistance to antibiotics.

不通常包菌选择性优势的基因,例如抗生素耐药性。

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Osmosis-微生物

The cytoplasm contains ribosomes which are the site of protein synthesis, the nucleoid which contains the genetic material, and plasmids which contain genes that confer a selective advantage to the bacterium.

胞中有核糖体,它们是蛋白合成的部位;拟核有遗传物以及;有菌选择性优势的基因。

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Osmosis-微生物

Plasmids have relatively few genes and their genetic information is not essential to the bacterium and cells that lack them usually function normally.

所的基因相对较少,并且它们的基因对菌来说并不是必须的,缺少的胞往往也可以正常生长。

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Osmosis-微生物

Additionally to the genetic material contained within the nucleoid, many bacteria contain extra chromosomal DNA molecules called plasmids.

除了包在拟核中的遗传物外,许多菌有额外的DNA分子,称为。

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Did You Know? – Verge Science

Then they dropped the plasmid into a living bacterium, or yeast.

然后他们将放入活菌或酵母中。

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Paper Talk

They loaded it with plasmid's DNA, carrying the CRR gene, and placed this gel right into the brain cavity after removing a glial blastoma tumor in mice.

他们将携带CRR基因的DNA装入其中,然后在小鼠切除胶母胞瘤后,将这种凝胶直接植入脑腔。

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SciShow 合集

But this is the first time the plasmid has been found in the United States, and it could get passed around quickly.

但这还是第一次在美国发现这种,它可能会迅速传播开来。

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2017级专外有声

Vectors also incorporate a selectable marker, a gene which allows host cells containing the vector to be selected from amongst those which do not, usually by conferring resistance to a toxin, or enabling their survival under certain growth conditions.

最初的大肠杆菌载体是染色体外的环形,并且相当数量的噬菌体(感染菌的病毒)也被用在大肠杆菌中。

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Life Noggin

So essentially, these plasmids are the vehicles that transfer genes for antibiotic resistance between different bacterial species.

因此本上,这些是在不同菌物种之间转移抗生素抗性基因的载体。

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语料库

Soon after, others began using plasmid vectors and adding genes for antibiotic resistance, greatly increasing the reach of the recombinant techniques. [81]

不久之后,其他人开始使用载体并添加抗生素抗性基因,大大增加了重组技术的应用范围[81]。

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Writing in the Sciences

This one had cell culture and transfections, antibodies, plasmids, recombinant virus production and infection, and so on and so forth.

这个有胞培养和转染、抗体、、重组病毒生产和感染等等。

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SciShow 合集

And the thing that makes this mutation especially troubling is that it was found on a plasmid -- a little piece of DNA that can be easily shared between neighboring bacteria, meaning it can spread more easily.

让这种突变特别令人担忧的是,它出现在一个上——这是一种可以轻易在邻近菌之间共享的小段DNA,这意味着它可以更容易地传播。

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Life Noggin

Or, in plain english, plasmids are part of the reason that, one day, antibiotics may not be able to help your strep throat anymore.

或者,用简单的英语来说,是有一天抗生素可能无法再帮助您治疗链球菌性咽喉炎的部分原因。

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博兹曼科学——生物

And so a plasmid, remember this would be the chromosomal DNA. This would be the chromosome of the bacteria.

因此,,记住这将是染色体DNA。这将是菌的染色体。

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2017级专外有声

More specialized vectors have been engineered to use aspects of plasmids and bacteriophages, such as the plasmid-bacteriophage h hybrids known as cosmids

和噬菌体已经在很多种类的菌中被使用来表达基因,并且一些噬菌体也许已经被用来将DNA整合到宿主基因组上。

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博兹曼科学——生物

We're just transferring that DNA, that plasmid from one to another.

我们只是将DNA,即,从一个转移到另一个。

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SciShow 合集

This plasmid mutation has been seen in other places before; in China, in animals and some human patients.

这种突变之前在其他地方出现;在中国,出现在一些动物和人类患者身上。

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