Now, Leptospira is a tiny spiral bacteria, that stains poorly with Gram staining, because it doesn't retain the Gram dyes well, so it's classically considered gram-negative.

钩端螺旋体是一种细小的螺旋，兰氏染色效果较差，因为它不能很好地保留染色剂，所以通常认为它是一种兰氏阴性。

Alright, as a quick recap, leptospira is a genus of tiny spiral gram-negative bacteria that can be seen under a darkfield microscope.

得！快速回一下：钩端螺旋体是一种细小的兰氏阴性，它可以在暗视野显微镜中被观察到。

This medium helps identify whether Gram-negative bacteria are lactose fermenters or not.

该类培养基有助于兰氏阴性是否为乳糖发酵。

It consists of peptidoglycan which usually helps differentiate whether bacteria are gram positive or gram negative.

细胞壁由肽聚糖组成，通常可以帮助区分细的种类，判断它们是兰氏阳性还是兰氏阴性。

They have a cell wall which protects the bacteria, determines whether they are gram positive or gram negative bacteria and also helps maintain their shape.

保护细的细胞壁决定了细是兰氏阳性还是兰氏阴性，并且参与维持细的状。

In gram negative bacteria it is internal and difficult for penicillin to access.

在兰氏阴性中，它位于内部，青霉素难以接触。

And I was dealing with things like candida overgrowth, gram-negative bacteria overgrowth.

我正在应对念珠过度生长、兰氏阴性过度生长等问题。