Single antibody of NSE, multi-antibody of glial fibrillary acidic protein (GFAP) and single antibody of neurofilament (NF200) were provided by Wuhan Boster Company.
METHODS The paratope mapping of antibodies was determined by competitive inhibition in radioimmunoassay and the sequences of variable regions among the antibodies were compared.
Objective To investigate the positive rate for irregulate erythrocyte antibodies by microtubes gel test(MGT) and moditying polybrene test(MPT),explore the value of MGT in clinic.
Information about their glomerular target antigens is inconsistent, and whether aailability of target antigens, antibody specificity or aidity are nephritogenic parameters, is not determined.
Conclusion:The purified fusion protein has good fineness and hemolysis activity, the antibody titers initiated by the protein vaccine go with regulation and the immunoprotection is satisfied.