Objective To develop a simple and effective m etho d for the separation and purification of chymopapain and study the zymological p roperty of the enzyme.

目的建立简单有效的木瓜凝乳蛋白酶分纯化法,研究其酶学质。法采用换层析直接将木瓜凝乳蛋白酶分纯化,通过温度、葡萄糖、盐酸胍及其他因素研究酶学质。

Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.

建立简单有效的木瓜凝乳蛋白酶分纯化法,研究其体外溶核作用。