Objective To develop a simple and effective m etho d for the separation and purification of chymopapain and study the zymological p roperty of the enzyme.

目的建有效的木瓜凝乳蛋白酶分方法,并研究其酶学质。方法采用子交换层析直接将木瓜凝乳蛋白酶分,通过温度、葡萄糖、盐酸胍及其他因素研究酶学质。

Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.

建有效的木瓜凝乳蛋白酶分方法,并研究其体外溶核作用。