Methods The sciatic and brachial plexus nerve of three-day-old SD mice was treated with collagenase type IV and trypsin to remove the desmocyte on the Schwann cell, then explanted in the culture disk.

先用IV酶和胰蛋白酶消化出生3天SD鼠臂层神经和坐骨神经组再将组贴壁培养。