The results showed that a mixed mating system was possibly the main heterogamy of this species, and its endangerment was caused by over-exploitation and habitat loss.

荷叶铁线蕨濒危的主要原因是生境的破坏以及所的生境片断化使其居群变小、近交率加大、遗传变异趋低，降低了其生存以及进化的潜力。