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Result Both mutanolysin and lysozyme could successfully lyse the wall of bifidobacterium and extract the plasmid from Bifidobacterium.

结果球菌发酵液与溶菌酶均取到质粒。

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Method Bifidobacterium plasmid was extracted by mutanolysin from Streptomyces globisporus which could lyse the wall of bifidobacterium.The result was compared with that by lysozyme.

法利用球菌发酵产生的变溶菌素来消化双歧杆菌细胞壁,取质粒,并与商业化溶菌酶法比较。

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Some are exchanged via small loops of DNA called plasmids.

一些基因会通过被称为质粒DNA小环交换。

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Osmosis-微生物

Additionally to the genetic material contained within the nucleoid, many bacteria contain extra chromosomal DNA molecules called plasmids.

除了包含在拟核中遗传物质外,许多含有额外DNA分子,称为质粒。

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Did You Know? – Verge Science

Then they dropped the plasmid into a living bacterium, or yeast.

然后他们将质粒放入活或酵母中。

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Osmosis-微生物

However, plasmids often contain genes that confer a selective advantage to the bacterium such as resistance to antibiotics.

不过质粒通常包含赋予选择性优势基因,生素耐药性。

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Osmosis-微生物

These plasmids are small double-stranded DNA molecules that can exist independently of the chromosome and they can be circular or linear.

它们是双链DNA小分子,可以独立于体(拟核)而存在,可以是环状或线状

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2017级专外有声

Transfer of the ligated plasmid into an E. coli strain (transformation).

7。转化成功筛选。

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Life Noggin

So essentially, these plasmids are the vehicles that transfer genes for antibiotic resistance between different bacterial species.

因此,从本质上讲,这些质粒是在不同物种之间转移生素性基因载体。

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SciShow 合集

This plasmid mutation has been seen in other places before; in China, in animals and some human patients.

这种质粒突变以前在其他地方也见过;在中国,在动物和一些人类患者中。

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Life Noggin

The plasmid enters and multiplies itself within a cell and can then be transferred to others.

质粒进入胞并在胞内繁殖, 然后可以转移到其他胞中。

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2017级专外有声

The cDNAs had to be linked to prokaryotic transcription and translation signals and inserted into multicopy plasmids.

然而,占总蛋白30%过量表达蛋白质是沉淀或不溶形式并且缺少翻译后修饰。

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Osmosis-微生物

Plasmids have relatively few genes and their genetic information is not essential to the bacterium and cells that lack them usually function normally.

质粒所含基因相对较少,并且它们基因对来说并不是必须,缺少质粒胞往往也可以正常生长。

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SciShow 合集

But this is the first time the plasmid has been found in the United States, and it could get passed around quickly.

但这是第一次在美国发现这种质粒,它可能会很快传播开来。

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2017级专外有声

More specialized vectors have been engineered to use aspects of plasmids and bacteriophages, such as the plasmid-bacteriophage h hybrids known as cosmids

质粒和噬体已经在很多种类中被使用来表达基因,并且一些噬体也许已经被用来将DNA整合到宿主基因组上。

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Life Noggin

Or, in plain english, plasmids are part of the reason that, one day, antibiotics may not be able to help your strep throat anymore.

或者,用简单英语来说,质粒是有一天生素可能无法再帮助您治疗链球性咽喉炎部分原因。

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2017级专外有声

Phage h can be used to clone fragments larger than plasmid vectors, and phage M13 allows cloned DNA to be isolated in single-stranded form.

更多载体被改造能同时拥有质粒和噬一些特性,像被称为粘粒质粒-噬体λ杂交体。

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2022诺贝尔得主访谈录

I did all these experiments actually, with my own hands, I was 58 years old, I was still culturing plasmids and feeding cells, so it is very unlikely.

实际上,所有这些实验都是我亲手做,当时我 58 岁,我还在培养质粒和喂养胞,所以这是非常不可能

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Osmosis-微生物

The cytoplasm contains ribosomes which are the site of protein synthesis, the nucleoid which contains the genetic material, and plasmids which contain genes that confer a selective advantage to the bacterium.

胞质中含有核糖体,它们是蛋白质合成部位;拟核含有遗传物质以及质粒;质粒含有赋予选择性优势基因。

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SciShow 合集

And the thing that makes this mutation especially troubling is that it was found on a plasmid -- a little piece of DNA that can be easily shared between neighboring bacteria, meaning it can spread more easily.

让这种突变特别麻烦是它是在质粒上发现——一小段 DNA 可以很容易地在相邻之间共享,这意味着它可以更容易地传播。

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