After absorption, prulifloxacin is metabolised by esterases to ulifloxacin, which inhibits the formation of superhelix, cell division and reproduction by interfering the DNA toposomerase.

本品为前体药,其活性代通过阻碍DNA拓扑构酶使细菌DNA无法超螺旋,导致细菌细胞无法分裂繁殖。