We separated the subfractions of LDL by precipitating method and density gradient ultracentrifugation, and identified the subfraction by electronphoresis and electron microscope.

通沉淀法分LDL,然超速法分亚组分。

Methods The cell-free system was prepared by ultracentrifugation of Xenopus eggs and activated by cytochrome C.

方法超速备非洲爪蟾卵无细胞体系,用细胞色素C激活为凋亡体系。