A method for simultaneous separation and determination of two coumarins,dicumarol and cyclocoumarol,in cosmetics by HPLC-diode-array detector(DAD) was developed.
Objective:HPLC was used to determine the contents of glycyrrhizic acid,isoliquiritin and isoliquiritigenin in compound liquorice tablets quantitatively.
So the absorbing wavelength is selected,the operating condition optimized,and the quantitative and qualitative determination method on Polyoxin B by HPLC-DAD established.
Gatifloxacin eyedrops was prepared with benzalkonium as bacteriostat,NaCl as osmotic-pressure regulating agent and PVP as thickener:The content was determined with HPLC.
The isomers dostribution of ortho-and para-toluenesulfonamide are successfully determined by high performance liquidchromatography (HPLC) . The results are compared with ultraviolet-spectroscopy (UV).
The results showed that the relative deviation of this method was less than 5% when compared with that of high-performance liquid chromatography, implying high accuracy of the presented biosensor in glucose detection in plants.