Gerlach and others had some luck using carmine to highlight different kinds of cell structures, but where Gerlach got stuck was in exploring the tissues of the brain.
But the waste product that these chemolithoautotrophs make is often in the form of minerals, like rust or pyrite, like fool's gold, or carminites, like limestone.
So one day, he tried making a diluted version of the stain — thinning out the carmine with ammonia and gelatin — and wetted a sample of brain tissue with it.
Back in his day, a few scientists had been tinkering with staining tissues, especially with a compound called carmine — a red dye derived from the scales of a crushed-up insects.
The carminic acid molecules are stored as clumps in her hemolymph – the insect equivalent of our blood – and ward off some would-be predators, like ants, and harmful microbes.
By its carmined lip, its near-smart hat, its babbling of " him, " and by the knowledge which looks boldly out of its eyes you know it is tragically old.
Each bug is about as big as a grain of rice, so you have to collect, dry, crush, and process tens of thousands of them for each kilogram of cochineal dye.