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Method Bifidobacterium plasmid was extracted by mutanolysin from Streptomyces globisporus which could lyse the wall of bifidobacterium.The result was compared with that by lysozyme.

方法利球孢链霉菌发酵变溶菌素来消化双歧杆菌细胞壁,提取质粒,并与商业化溶菌酶法比较。

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NPC cell line (CNE1) as target,Electroporation was used to transfect EBV-LMP gene and the vector into CNE1 cells.

以人高分化鼻咽癌细胞株(CNE1)为对象,穿孔基因转染技术,将重组EBV-LMP表达质粒转染CNE1细胞。

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Result Both mutanolysin and lysozyme could successfully lyse the wall of bifidobacterium and extract the plasmid from Bifidobacterium.

结果球孢链霉菌发酵液与溶菌酶均成功提取到质粒

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经济学人-科技

Some are exchanged via small loops of DNA called plasmids.

一些基因会通过被称为质粒DNA交换。

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博兹曼科学——

They also have extra little tiny loops called plasmids.

它们还具有额外,称为质粒

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2022诺贝尔得主访谈录

I did all these experiments actually, with my own hands, I was 58 years old, I was still culturing plasmids and feeding cells, so it is very unlikely.

实际上,所有这些实验都是我亲手做,当时我 58 岁,我还在培养质粒和喂养细胞,所以这是非常不可能

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Osmosis-微

However, plasmids often contain genes that confer a selective advantage to the bacterium such as resistance to antibiotics.

不过质粒通常包赋予细菌选择性优势基因,例如抗素耐药性。

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Osmosis-微

The cytoplasm contains ribosomes which are the site of protein synthesis, the nucleoid which contains the genetic material, and plasmids which contain genes that confer a selective advantage to the bacterium.

细胞质中有核糖体,它们是蛋白质合成部位;拟核有遗传质以及质粒质粒有赋予细菌选择性优势基因。

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Osmosis-微

Plasmids have relatively few genes and their genetic information is not essential to the bacterium and cells that lack them usually function normally.

质粒基因相对较少,并且它们基因对细菌来说并不是必须,缺少质粒细胞往往也可以正常长。

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Osmosis-微

Additionally to the genetic material contained within the nucleoid, many bacteria contain extra chromosomal DNA molecules called plasmids.

了包在拟核中遗传质外,许多细菌有额外DNA分子,称为质粒

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Did You Know? – Verge Science

Then they dropped the plasmid into a living bacterium, or yeast.

然后他们将质粒放入活细菌或酵母中。

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Paper Talk

They loaded it with plasmid's DNA, carrying the CRR gene, and placed this gel right into the brain cavity after removing a glial blastoma tumor in mice.

他们将携带CRR基因质粒DNA装入其中,然后在鼠切胶质母细胞瘤后,将这种凝胶直接植入脑腔。

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SciShow 合集

But this is the first time the plasmid has been found in the United States, and it could get passed around quickly.

但这还是第一次在美国发现这种质粒,它可能会迅速传播开来。

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2017级专外有声

Vectors also incorporate a selectable marker, a gene which allows host cells containing the vector to be selected from amongst those which do not, usually by conferring resistance to a toxin, or enabling their survival under certain growth conditions.

最初大肠杆菌载体是染色体外质粒,并且相当数量噬菌体(感染细菌病毒)也被用在大肠杆菌中。

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Life Noggin

So essentially, these plasmids are the vehicles that transfer genes for antibiotic resistance between different bacterial species.

因此本质上,这些质粒是在不同细菌种之间转移抗素抗性基因载体。

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语料库

Soon after, others began using plasmid vectors and adding genes for antibiotic resistance, greatly increasing the reach of the recombinant techniques. [81]

不久之后,其他人开始使用质粒载体并添加抗素抗性基因,大大增加了重组技术应用范围[81]。

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Writing in the Sciences

This one had cell culture and transfections, antibodies, plasmids, recombinant virus production and infection, and so on and so forth.

这个有细胞培养和转染、抗体、质粒、重组病毒产和感染等等。

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SciShow 合集

And the thing that makes this mutation especially troubling is that it was found on a plasmid -- a little piece of DNA that can be easily shared between neighboring bacteria, meaning it can spread more easily.

让这种突变特别令人担忧是,它出现在一个质粒上——这是一种可以轻易在邻近细菌之间共享段DNA,这意味着它可以更容易地传播。

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Life Noggin

Or, in plain english, plasmids are part of the reason that, one day, antibiotics may not be able to help your strep throat anymore.

或者,用简单英语来说,质粒是有一天抗素可能无法再帮助您治疗链球菌性咽喉炎部分原因。

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博兹曼科学——

And so a plasmid, remember this would be the chromosomal DNA. This would be the chromosome of the bacteria.

因此,质粒,记住这将是染色体DNA。这将是细菌染色体。

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2017级专外有声

More specialized vectors have been engineered to use aspects of plasmids and bacteriophages, such as the plasmid-bacteriophage h hybrids known as cosmids

质粒和噬菌体已经在很多种类细菌中被使用来表达基因,并且一些噬菌体也许已经被用来将DNA整合到宿主基因组上。

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博兹曼科学——

We're just transferring that DNA, that plasmid from one to another.

我们只是将DNA,即质粒,从一个转移到另一个。

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SciShow 合集

This plasmid mutation has been seen in other places before; in China, in animals and some human patients.

这种质粒突变之前在其他地方出现过;在中国,出现在一些动和人类患者身上。

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