Objective To investigate the feasibility of applying immunohistochemistry (IH) and in situ hybridization (ISH) technique to the undecalcified bone sections.
It is shown that expansible ettringite forms in the topochemical reaction,and crystallizes in-situ on the surface of unhydrated aluminate minerals and orients radially.
The increase in calcium current occurred within 1 minute of superfusion and the sustained inward holding current was slowly activated after prominent cell swelling.
The results showed: inncrease application of Ca, balance application of N and K and sprinkler irrigation could increase Ca content of lettuce leaves, reduce occurring of tipburn.