preincubation

Preincubation of the cultures with ciliary neurotrophic factor (CNTF) for 24 h promoted neuronal survival and decreased the LDH efflux and the level of NO induced by ionomycin.

提前24 h给予不同浓度CNTF, 均能提高神经元的存, 少LDH漏出, 其作用呈剂量依赖性;CNTF可降低ionomycin引起的NO含量升高;

The ovarian follicles of 600-700μm in diameter responsed to 5 and 10 ng/ml of 17α,20β-DHP and underwent oocyte GVBD after 3 or 9 of hours preincubation with 50 IU/ml HCG.

经3或9小时50 IU/ml 绒毛膜促性腺激素预孵育后，600-700μm的离体卵泡能对5和10 ng/ml的17α,20β-双羟孕酮起发生胚泡破裂。