Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.
法 标本组织块用冷消化法离出尿路上皮,培于无血清生长培。
Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.
法 标本组织块用冷消化法离出尿路上皮,培于无血清生长培。
Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.
法取大鼠胚离成纤维,别采用悬液法和胰酶消化植块培法对其进行培。
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