Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.
方法 标本组织块用冷
化法分离出尿路上
，培养于无血清生长培养基。
蛋白
化(作用)Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.
方法 标本组织块用冷化法分离出尿路上，培养于无血清生长培养基。
Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.
方法取大鼠胚
分离成纤维,分别采用悬液法和
化植块培养法对其进行培养。
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