Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.
建立简单有效的木
凝乳蛋白酶分离纯化方法,并研究其体外溶核作用。
凝乳蛋白酶Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.
建立简单有效的木凝乳蛋白酶分离纯化方法,并研究其体外溶核作用。
Objective To develop a simple and effective m etho d for the separation and purification of chymopapain and study the zymological p roperty of the enzyme.
目的建立简单有效的木凝乳蛋白酶分离纯化方法,并研究其酶学性质。方法采用离子交换层析直接将木凝乳蛋白酶分离纯化,通过温度、葡萄糖、盐酸胍及其
研究酶学性质。
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