Result Both mutanolysin and lysozyme could successfully lyse the wall of bifidobacterium and extract the plasmid from Bifidobacterium.
结果球孢链霉菌发酵液与溶菌酶均成功提取到粒。
Result Both mutanolysin and lysozyme could successfully lyse the wall of bifidobacterium and extract the plasmid from Bifidobacterium.
结果球孢链霉菌发酵液与溶菌酶均成功提取到粒。
NPC cell line (CNE1) as target,Electroporation was used to transfect EBV-LMP gene and the vector into CNE1 cells.
以人高分化鼻咽癌细胞株(CNE1),采用电穿孔基因转染技术,将重组EBV-LMP表达粒转染CNE1细胞。
Method Bifidobacterium plasmid was extracted by mutanolysin from Streptomyces globisporus which could lyse the wall of bifidobacterium.The result was compared with that by lysozyme.
方法利用球孢链霉菌发酵变溶菌素来消化双歧杆菌细胞壁,提取粒,并与商业化溶菌酶法比较。
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